HAC (Russian)
RSCI (Russian)
Ulrichsweb (Ulrich’s Periodicals Directory)
Scientific Indexing Services

Biomarkers of bone and cartilage remodeling in the evaluation of early stages of primary knee osteoarthritis

DOI: https://doi.org/10.29296/25877305-2022-10-18

S. Belova, Biol.D; E. Gladkova, Candidate of Biological Sciences; R. Zubavlenko; Associate Professor V. Ulyanov, MD
Research Institute of Traumatology, Orthopedics, and Neurosurgery,
V.I. Razumovsky Saratov State Medical University, Ministry of Health of Russia

Objective. To determine the informative value of biological markers of bone and cartilage remodeling in patients with early-stage primary knee osteoarthritis (KOA). Subject and methods. The investigation involved 43 patients (16 men and 27 women) aged 36–50 years with early manifestations of KOA and 21 apparently healthy individuals without locomotor diseases. The markers of bone tissue metabolism were determined in all the study participants: resorptive processes were assessed by the content of C-terminal telopeptides of type I collagen and pyridinoline; the intensity of bone formation processes was estimated by the level of osteocalcin. The concentrations of cartilage oligomeric matrix protein and cartilage glycoprotein were determined as markers of cartilage destruction. Results. The significantly elevated levels of cartilage oligomeric matrix protein and cartilage glycoprotein show evidence of articular cartilage disorganization in the examinees. In addition, there were impaired bone formation (higher osteocalcin levels) and resorption (an increase in the concentrations of C-terminal telopeptides of type I collagen and pyridinoline) processes compared with those in the control group. Conclusion. The patients with early signs of KOA without obvious clinical and radiographic manifestations were observed to have impaired bone remodeling as intensified bone formation and resorption processes, which was accompanied by cartilage matrix restructuring. An indirect assessment of the stability of the collagen network that forms the extracellular framework of the supporting connective tissues displayed a significant increase in serum pyridinoline concentrations, which could be evidence of disruption of intermolecular collagen bonds. The reliably significant changes in the content of pyridinoline suggest that this marker may be used to comprehensively evaluate the health status of patients with early manifestations of KOA when developing diagnostic and therapeutic strategies.

primary osteoarthritis
knee joints
early stage
bone and cartilage remodeling

  1. Isleiikh O.I. Vnutrikostnoe vvedenie autologichnoi obogashchennoi trombotsitami plazmy v lechenii gonartroza. Diss. ... kand. med. nauk. M., 2020; 146 р. (in Russ.).
  2. Alekseeva L.I., Zaitseva E.M. Subkhondral'naya kost' pri osteoartroze: novye vozmozhnosti terapii. RMJ. 2004; 20: 1133 (in Russ.).
  3. Hilal G., Martel-Pelletier J., Pelletier J.P. et al. Osteoblast-like cell from human subchondral osteoarthritic bone demonstrate an altered phenotype in vitro: possible role in subchondral bone sclerosis. Arthritis Rheum. 1998; 41 (5): 891–9. DOI: 10.1002/1529-0131(199805)41:53.0.CO;2-X
  4. Kabalyk M.A. Biomarkers of subchondral bone remodeling in osteoarthritis. Pacific Medical Journal. 2017; 1: 36–41 (in Russ.). DOI: 10.17238/PmJ1609-1175.2017.1.37-41
  5. Igarashi M., Sakamoto K., Nagaoka I. Effect of glucosamine, a therapeutic agent for osteoarthritis, on osteoblastic cell differentiation. Int J Mol Med. 2011; 28 (3): 373–9. DOI: 10.3892/ijmm.2011.686
  6. Belova Yu., Gladilin E. Diagnostic value of cartilage oligomeric matrix protein in connective tissue disease. Vrach. 2017; 10: 83–5 (in Russ.).
  7. Ktsoeva A.A. Klinicheskoe znachenie khryashchevogo glikoproteina-39 u bol'nykh osteoartritom v sochetanii s kardiovaskulyarnoi patologiei. Avtoref. … kand. med. nauk. Volgograd, 2019; 24 р. (in Russ.).
  8. Kraus V.B., Nevitt M., Sandell L.J. Summary of the OA biomarkers workshop 2009-biochemical biomarkers: biology, validation, and clinical studies. Osteoarthritis Cartilage. 2010; 18 (6): 742–5. DOI: 10.1016/j.joca.2010.02.014
  9. Shishkova V.N. Osteoporosis in neurological: practice: focus on the spine. Farmateka. 2013; 9: 24–8 (in Russ.).
  10. Gallop P.M., Blumenfeld O.O., Seifter S. Structure and metabolism of connective 801 tissue proteins. Annu Rev Biochem. 1972; 41: 617–72. DOI: 10.1146/annurev.bi.41.070172.003153
  11. Lindert U., Kraenzlin M., Campos-Xavier A.B. et al. Urinary pyridinoline cross-links as biomarkers of osteogenesis imperfecta. Orphanet J Rare Dis. 2015; 10: 104. DOI: 10.1186/s13023-015-0315-9
  12. Ilina R.Yu., Mukhamedzhanova L.R., Urakova E.V. Change of biochemical markers of bone tissue metabolism on the background of medicine osteoporosis in mental patients. Prakticheskaya meditsina. 2013; 2: 63–6 (in Russ.).
  13. Krabben A., Knevel R., Huizinga T.W.J. et al. Serum pyridinoline levels and prediction of severity of joint destruction in rheumatoid arthritis. J Rheumatol. 2013; 40 (8): 1303–6. DOI: https://doi.org/10.3899/jrheum.121392
  14. Takahashi M., Naito K., Abe M. et al. Relationship between radiographic grading of osteoarthritis and the biochemical markers for arthritis in knee osteoarthritis. Arthritis Res Ther. 2004; 6 (3): 208–12. DOI: 10.1186/ar1166
  15. Li Q., Hu L., Zhao Z. et al. Serum changes in pyridinoline, type II collagen cleavage neoepitope and osteocalcin in early stage male brucellosis patients. Sci Rep. 2020; 10: 17190. DOI: 10.1038/s41598-020-72565-8