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THE ROLE OF MOLECULAR GENETIC METHODS IN THE DETECTION OF TUBERCULOSIS IN THE GENERAL HEALTHCARE NETWORK

DOI: https://doi.org/10.29296/25877305-2022-10-07
Issue: 
10
Year: 
2022

A. Kolesnikova(1), Professor B. Borodulin(1), MD; D. Kudlay(2, 3), Corresponding Member of RAS, MD Associate Professor L. Povalyaeva(1), Candidate of Medical Sciences
1-Samara State Medical University, Ministry of Health of Russia
2-I.M. Sechenov First Moscow State Medical University (Sechenov University), Ministry of Health of Russia
3-NRC Institute of Immunology FMBA of Russia, Moscow

The problem of timely tuberculosis (TB) detection remains relevant even when its epidemic situation improves. Despite a well-organized preventive examination system, many TB cases are detected when a patient visits the general medical network. This problem is most often faced by a pulmonologist especially at the stage of inpatient treatment. The implementation of the diagnostic minimum in the inpatient pulmonology department is a routine practice. When communicating with each pulmonology patient, the physician should be alert to TB when collecting historical data, examining, planning an examination, by obligatorily using the current diagnostic methods. This review considers the role of a pulmonologist in detecting TB when patients visit the general medical network. A rationale is provided for the necessity of including rapid molecular genetic technologies into the standard diagnostic minimum in order to detect Mycobacterium tuberculosis DNA in patients at risk for TB.

Keywords: 
phthisiology
tuberculosis
pulmonologist
Mycobacterium tuberculosis
diagnostic minimum
general medical network
tuberculosis risk groups



References: 
  1. Vаsilyevа I.A., Belilovsky E.M., Borisov S.E., Sterlikov S.A. Incidence, mortality and prevalence as indicators of tuberculosis burden in who regions, countries of the world and the Russian Federation. part 1. tuberculosis incidence and prevalence. Tuberculosis and Lung Diseases. 2017; 95 (6): 9–21 (in Russ.). DOI: 10.21292/2075-1230-2017-95-6-9-21
  2. Ergeshov АЕ. Tuberculosis in the Russian Federation: Situation, Challenges and Perspectives. Annals of the Russian Academy of Medical Sciences. 2018; 73 (5): 330–7 (in Russ.). DOI: 10.15690/vramn1023
  3. Borodulin B.E., Borodulina E.A., Eremenko E.P. Ambulatornaya ftiziatriya. M., 2022; 420 р. (in Russ.).
  4. Zubova N.A., Tsybikova E.B., Punga V.V. et al. Pulmonary tuberculosis patients detected during mass screening and by referral to medical units. Tuberculosis and Lung Diseases. 2016; 94 (11): 22–8 (in Russ.). DOI: 10.21292/2075-1230-2016-94-11-22-28
  5. Guseynalieva V.N. Improved detection of tuberculosis in primary health care and its impact on the incidence. Tuberculosis and Lung Diseases. 2020; 98 (10): 41–6 (in Russ.). DOI: 10.21292/2075-1230-2020-98-10-41-46
  6. Djumaniazova G.M., Bekсhanova B.B. Tuberculosis in thе practice of а роliclinic doctor. European Science. 2020; 1: 73–6 (in Russ.).
  7. Tashpulatova F.K., Ziyamukhamedov A.N.U., Ibragimova Kh.O.K. Vyyavlenie tuberkuleza legkikh v obshchesomaticheskikh lechebnykh uchrezhdeniyakh. Molodoi uchenyi. 2017; 3: 236–8 (in Russ.).
  8. Borodulina E.A., Inkova A.T., Borodulin B.E. и др. Ways to optimize detection of tuberculosis in a pulmonology unit. Tuberculosis and Lung Diseases. 2018; 96 (5): 22–6 (in Russ.). DOI: 10.21292/2075-1230-2018-96-5-22-26
  9. Dubrovskaya I.I., Bagisheva N.V., Mordyk A.V. et al. Detection and differential diagnosis of tuberculosis in patients of the pulmonary department with community-acquired pneumonia and chronic obstructive pulmonary disease. Pulmonologiya. 2020; 30 (3): 305–11 (in Russ.). DOI: 10.18093/0869-0189-2020-30-3-305-311
  10. Borodulina E.V., Borodulina E.A., Gladunova E.Р. et al. Medico-social characteristics of newly diagnosed patients with tuberculosis. Meditsinskii al'yans. 2018; 3: 41–6 (in Russ.).
  11. Sevastyanova E.V., Chernousova L.N. Modern algorithms of microbiological diagnostics of tuberculosis. Tuberculosis and Lung Diseases. 2018; 96 (7): 11–7 (in Russ.). DOI: 10.21292/2075-1230-2018-96-7-11-17
  12. Seregina V.A., Budritsky A.M. Modern possibilities of pulmonary tuberculosis diagnosing. Vestnik VGMU. 2016; 15 (4): 7–17 (in Russ.).
  13. Dogadaeva E., Lopatina T., Sokolov V. Tuberculosis diagnostic features. Vrach. 2020; 31 (10): 74–84 (in Russ.). DOI: 10.29296/25877305-2020-10-14
  14. Kim T.M., Chubakov T.Ch., Dushimbekova K.A. et al. Comparative assessment of the early tb detection methods. Vestnik poslediplomnogo obrazovaniya v sfere zdravookhraneniya. 2017; 3: 21–24 (in Russ.).
  15. Rodionova Yu.D., Gusyakova O.A., Lyamin A.V. et al. Evaluation of the effect of sputum storage conditions on the vital properties of tuberculous mycobacteria. Tuberculosis and Lung Diseases. 2017; 95 (1): 42–6 (in Russ.). DOI: 10.21292/2075-1230-2017-95-1-42-46
  16. Pyanzova T.V., Dzhangildin Yu.T., Vasilyeva I.А. Models of сlinical situations – a new methodological approach to analysis of patient populations in TB facilities. Tuberculosis and Lung Diseases. 2022; 100 (4): 41–5 (in Russ.). DOI: 10.21292/2075-1230-2022-100-4-41-45
  17. Kerefova Z.Sh., Baisultanova F.Kh., Pshegusova M.Kh. Sovremennye aspekty klinicheskoi kartiny tuberkuleza legkikh. Novaya nauka: Teoreticheskii i prakticheskii vzglyad. 2016; 117: 48–53 (in Russ.).
  18. Karpina N.L. The peculiarities of differential diagnosis of pulmonary diseases characterized by infiltration. CTRI Bulletin. 2018; 1: 26–3 (in Russ.). DOI: 10.7868/S2587667818010041
  19. Gaydarov G.M., Aphanova N.S. The comprehensive organization of identification of tuberculosis in population of the Eastern Siberian Region. Problemi socialnoi gigieni, zdravookhranenia i istorii meditsini. 2020; 28 (6): 1270–4 (in Russ.). DOI: 10.32687/0869-866X-2020-28-6-1270-1274
  20. Drobot N.N., Kondratyev E.G., Zinnurova L.A. et al. Cross-disciplinary competences – the basis of high-quality diagnostics and treatment of tuberculosis. International Journal Of Applied And Fundamental Research. 2016; 11: 896–9 (in Russ.).
  21. Punin A.A., Guseva I.I., Korotkova E.A. et al. Detection of tuberculosis cases among the patients of somatic hospital. Vestnik Smolenskoi gosudarstvennoi meditsinskoi akademii. 2016; 4: 58–63 (in Russ.).
  22. Kuday D.A. Biomarkers and immunological tests. Experimental and clinical parallels of latent tuberculosis infection. Tuberculosis and Lung Diseases. 2020; 98 (8): 63–74 (in Russ.). DOI: 10.21292/2075-1230-2020-98-8-63-74
  23. Aksenova V.A., Levi D.T., Aleksandrova N.V. et al. Pediatric TB: Modern Methods for Prevention and Early Diagnostics. Doctor.Ru. 2017; 15 (144): 9–15 (in Russ.).
  24. Kudlay D.A., Starshinova A.A., Dovgalyuk I.F. Recombinant tuberculosis allergen: 10 years of experience with the test in children and adolescents in the Russian Federation (meta-analysis data). Pediatria n.a. G.N. Speransky. 2020; 99 (3): 121–9 (in Russ.). DOI: 10.24110/0031-403X-2020-99-3-121-129
  25. Borodulina E.A., Kudlay D.A., Kuznetsova A.N. et al. The use of the ELISPOT technological platform in the diagnosis of tuberculosis infection in patients with HIV infection. Immunologiya. 2021; 42 (4): 395–402 (in Russ.). DOI: 10.33029/0206-4952-2021-42-4-395-402
  26. Aksenova V.A., Klevno N.I., Baryshnikova L.A. et al. Vyyavlenie tuberkuleza i taktika dispansernogo nablyudeniya za litsami iz grupp riska s ispol'zovaniem rekombinantnogo tuberkuleznogo allergena – Diaskintest®. Metodicheskie rekomendatsii. M.: Pervyi MGMU im. I.M. Sechenova, 2011; 12 р. (in Russ.).
  27. Sysoev P.G., Fattakhova E.N., Gubaidullina G.Z. et al. Otsenka svoevremennosti vyyavleniya tuberkuleza u bol'nykh s legochnym porazheniem. Sinergiya Nauk. 2018; 22: 1151–5 (in Russ.).
  28. Borodulina E.A., Matkina T.N., Vdoushkina E.S. Prichiny smerti bol'nykh VICh-infektsiei i tuberkulezom. Tuberculosis and Lung Diseases. 2015; 5: 44–5 (in Russ.).
  29. Panchenko I.N., Gavrilov P.V. Rol' profilakticheskikh flyuorograficheskikh obsledovanii v rannem vyyavlenii tuberkuleza v Respublike Komi. Tubinform. 2017; 1: 18–22 (in Russ.).
  30. Shubina A.T., Borodulina E.A., Kudlay D.A. et al. Test with recombinant tuberculosis allergen (Diaskintest®) in the diagnosis of pulmonary tuberculosis in pulmonological practice. Pulmonologiya. 2022; 32 (4): 576–84 (in Russ.). DOI: 10.18093/0869-0189-2022-32-4-576-584
  31. Sevastyanova E., Larionova E., Smirnova T. et al. Evaluation of the results of mycobacterium detection, obtained by different studies methods. Meditsinskii al'yans. 2018; 3: 25–30 (in Russ.).
  32. Kosulnicova A.S. Modern methods of diagnostics of the tuberculosis. Russian Military Medical Academy Reports. 2018; 37 (S 1-1): 347–50 (in Russ.).
  33. Artem'ev A.A. Defekty vedeniya bol'nykh tuberkulezom na etape okazaniya pervichnoi meditsinskoi pomoshchi. Bulletin of Medical Internet Conferences. 2016; 5: 1034 (in Russ.).
  34. Borodulina E.A., Shubina A.T., Gerasimov A.N. et al. GX technologies for rapid diagnosis of tuberculosis in general healthcare network. The Bulletin of Contemporary Clinical Medicine. 2022; 15 (1): 7–16 (in Russ.). DOI: 10.20969/VSKM.2022.15(1).7-16
  35. Vladimirov K.B., Marfina G.Yu., Karaseva E.V. et al. Etiological verification of tuberculosis in case of system inflammatory syndrome in HIV patients. Tuberculosis and Lung Diseases. 2019; 97 (3): 26–30 (in Russ.). DOI: 10.21292/2075-1230-2019-97-3-26-30
  36. Pavlov M.R. Biological properties of causative agent of tuberculosis, modern methods of its diagnosis. Russian Military Medical Academy Reports. 2019; S1: 52–6 (in Russ.).
  37. Artem'ev A.A. Prichiny formirovaniya lekarstvennoi ustoichivosti MBT u bol'nykh tuberkulezom. Bulletin of Medical Internet Conferences. 2017; 6: 1325 (in Russ.).
  38. Borodulina E.A., Povalyaeva L.V., Borodulina E.V. et al. Problem of tuberculous detection in pulmonologist's practice. The Bulletin of Contemporary Clinical Medicine. 2017; 10 (1): 89–93 (in Russ.). DOI: 10.20969/VSKM.2017.10(1).89-93
  39. Shugаevа S.N., Sаvilov E.D., Koshkinа O.G. et al. Impact of HIV infection on the intensity of tuberculosis epidemics on the territories of high risks for both infections. Tuberculosis and Lung Diseases. 2018; 96 (2): 5–10 (in Russ.). DOI: 10.21292/2075-1230-2018-96-2-5-10
  40. Borodulina Е., Vdoushkina Е., Inkovа А. Tuberculosis and HIVinfection. Vrach. 2020; 31 (1): 37–42 (in Russ.). DOI: 10.29296/25877305-2020-01-07
  41. Shilova M.V. Tuberculosis in Russia. Mortality of population from tuberculosis. Medical alphabet. 2018; 1 (10): 42–50 (in Russ.).
  42. Panteleev A.M., Nikulina O.V., Dracheva M.S. et al. Criteria of modern tuberculosis diagnostics in HIV-infection patients. Meditsinskiy sovet = Medical Council. 2016; 10: 120–4. (in Russ.). DOI: 10.21518/2079-701X-2016-10-120-124
  43. Zimina V.N., Mikova O.E., Varetskaya T.A. et al. Detection of mycobacterium tuberculosis in sputum of patients with HIV infection using a modern algorithm of etiological diagnosis of disease. Infectious Diseases. 2018; 1: 28–34 (in Russ.). DOI: 10.20953/1729-9225-2018-1-28-34
  44. Viktorova I.B., Degtyareva S.Yu., Kulabukhova E.I. et al. Detection of Mycobacterium tuberculosis in sputum in patients coinfected with HIV / tuberculosis by various methods (literature review). Journal Infectology. 2018; 10 (2): 30–8 (in Russ.). DOI: 10.22625/2072-6732-2018-10-2-30-38
  45. Eliseev P.I., Detjen A., Dacombe R. et al. Impact of molecular genetic methods on the initiation of chemotherapy in multiple drug resistanttuberculosis patients in Arkhangelsk region. Tuberculosis and Lung Diseases. 2017; 95 (12): 10–7 (in Russ.). DOI: 10.21292/2075-1230-2017-95-12-10-17
  46. Tsybikova E.D, Syunyakova D.A. Multy-drug resistant TB and extensively resistant TB: WHO materials in publications of the russian authors. Social aspects of population health [serial online]. 2019; 65 (3): 9. (in Russ.). DOI: 10.21045/2071-5021-2019-65-3-9