Development of a diagnostic panel of biomarkers for predicting the survival rate of dental implants in patients of older age groups

DOI: https://doi.org/10.29296/25877305-2022-06-16
Issue: 
6
Year: 
2022

A. Polyakova(1), Professor D. Medvedev(2), MD; V. Polyakova(3, 4), Biol.D; Professor of the Russian Academy of Sciences
1-Saint Petersburg State Pediatric Medical University, Ministry of Health of Russia
2-Research Institute of Hygiene, Occupational Diseases and Human Ecology, Federal Biomedical Agency of Russia, Kuzmolovsky Urban Settlement, Leningrad Region
3-Saint Petersburg Research Institute of Phthisiopulmonology, Ministry of Health of Russia
4-Belgorod State National Research University

The development of a diagnostic panel of biomarkers to improve the survival rate of dental implants is an urgent scientific and applied problem. The recovery rate for bone tissue innervation and vascularization is one of the important criteria for the success of implantation. Objective. To develop a diagnostic panel of biomarkers to assess the success of implantation in patients of older age groups Subjects and methods. The buccal epithelium was investigated in people of different ages. The sample size was 210 patients (5 age groups) and 420 observations (before and after implantation). Results. It was found that with increasing age, there were significant decreases in the expression level of the vascularization markers VEGF, VEGFR1 before implantation and in the level of the marker for the rate of innervation recovery in the implantation zone of NO synthase before and after implantation. After implantation, the level of VEGFR1 expression decreased in all studied groups; that of VEGF expression increased in all the groups, except for the group of long-livers, in whom the level of VEGF reduced after implantation compared to that before implantation. Conclusion. This investigation makes it possible to approach the solution of the problem of creating a differential diagnostic model to predict the survival rate of implants and to choose a habilitation method. The development and implementation of a diagnostic panel of biomarkers will be able to proceed to personalized medicine, high-tech healthcare, and health-saving technologies.
Keywords: 
geriatrics
dentistry
implantation
biomarkers
diagnostic panel of biomarkers

References: 
  1. Boichuk N.V., Chelyshev Yu.A. Gistologiya organov polosti rta: uchebno-metodicheskoe posobie. Kazan: KGMU, 2011; 96 s. (in Russ.).
  2. Bykov V.L. Funktsional'naya morfologiya epitelial'nogo bar'era slizistoi obolochki polosti rta. Stomatologiya. 1997; 76 (3): 12–7 (in Russ.).
  3. Bykov V.L. Tissue engineering of the oral mucosa. Morfologiya. 2010; 137 (1): 62–70 (in Russ.).
  4. Ortopedicheskaya stomatologiya: uchebnik. Pod red. I.Yu. Lebedenko, E.S. Kalivradzhiyana. M.: GEOTAR-Media, 2016; 640 s. (in Russ.).
  5. Paltsev M.A., Kvetnoy I.M., Polyakova V.O. et al. Signaling molecules in buccal epithelium: optimization of diagnosis of socially significant diseases. Molecular medicine. 2012; 2: 18–23 (in Russ.).
  6. Polyakova A.A. Metod kletochnoi biologii v realizatsii klinicheskikh zadach: otsenka prizhivaemosti implantov u pozhilykh patsientov. V kn.: VIII Mezhdunarodnaya nauchno-prakticheskaya konferentsiya molodykh uchenykh: biofizikov, biotekhnologov, molekulyarnykh biologov i virusologov – 2021. Sb. tez. konf., prokhod. v ramkakh ploshchadki otkrytykh kommunikatsii OpenBio-2021. Novosibirsk, 2021; s. 324. (in Russ.).
  7. Polyakova V.O., Pal'tseva E.M., Krulevskii V.A. Bukkal'nyi epitelii. Novye podkhody k molekulyarnoi diagnostike sotsial'no znachimoi patologii. SPb: Izd-vo N-L, 2015 (in Russ.).
  8. Simanenkov V.I., Maev I.V., Tkacheva O.N. et al. Syndrome of increased epithelial permeability in clinical practice. Multidisciplinary national Consensus. Cardiovascular Therapy and Prevention. 2021; 20 (1): 2758. (in Russ.). DOI: 10.15829/1728-8800-2021-2758
  9. Shakhbazov V.G., Grigor'eva N.N., Kolupaeva T.V. Novyi tsitobiologicheskii pokazatel' biologicheskogo vozrasta i biologicheskogo sostoyaniya organizma cheloveka. Fiziologiya cheloveka. 1996; 22 (6): 71–5 (in Russ.).
  10. Carbone A., Linkova N., Polyakova V. et al. Melatonin and Sirtuins in Buccal Epithelium: Potential Biomarkers of Aging and Age-Related Pathologies. Int J Mol Sci. 2020; 21 (21): 8134. DOI: 10.3390/ijms21218134
  11. Polyakova A., Medvedev D., Semiglazova J. et al. Buccal epithelium: as an object of non-invasive diagnostics of implant survival in people of different ages. Eur J Clin Invest Suppl. 2021; 51 (S1): 123–4.
  12. Saba R., Halytskyy O., Saleem N. et al. Buccal Epithelium, Cigarette Smoking, and Lung Cancer: Review of the Literature. Oncology. 2017; 93 (6): 347–53. DOI: 10.1159/000479796