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Possibilities of using the rs2070744 polymorphism of the endothelial nitric oxide synthase gene for the identification of severe coronary atherosclerosis in young and middle-aged patients with myocardial infarction

DOI: https://doi.org/10.29296/25877305-2020-09-06
Issue: 
9
Year: 
2020

E. Shishkina, Candidate of Medical Sciences; Professor O. Khlynova, MD, Corresponding Member of
the Russian Academy of Sciences; Professor A. Tuev, MD Acad. E.A. Vagner Perm State Medical University,
Ministry of Health of Russia

Analysis of genetic predisposition to coronary atherosclerosis in young and middle-aged patients is the subject of an urgent scientific search. Objective: to study the relationship of the rs2070744 polymorphic variants of the endothelial nitric oxide synthase (eNOS) gene to the severity of coronary atherosclerosis (CA). Subjects and methods. Examinations were made in 98 patients with myocardial infarction (MI), including 13 women and 85 men; their median age was 49.01 (44; 55) years. The T786C (rs2070744) polymorphism of the eNOS gene was determined by an allele-specific polymerase assay using the test systems manufactured by the OOO «Sintol» (Moscow). The severity of coronary bed lesions was assessed from coronary angiography readings, by calculating the Gensini score. To assess the association of alleles and genotypes with the severity of CA, the odds ratio (OR) was estimated with a 95% confidence interval (CI). Results. An association was shown between the carriage of the T/C genotype of the eNOS gene and the Gensini score in young and middle-aged patients with MI (r=0.325; p=0.013). In the group of patients with severe CA (Gensini scores ≥33), there were significantly more carriers of the T/C genotype (71.8% versus 35.3%; p=0.031; OR=4.67; 95% CI, 1.38–15.37) and the combined T/C + C/C genotype (79.5% versus 47.1%; p=0.015; OR=4.36; 95% CI, 1.28–14.9). Conclusion. The development of severe CA in young and middle-aged patients is genetically determined and associated with the carriage of the T/C and T/C + C/C genotypes of eNOS T786C polymorphism.

Keywords: 
cardiology
polymorphism
genetics
myocardial infarction
endothelial nitric oxide synthase gene
coronary atherosclerosis
young and middle age



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