PEPTIDES OF CARTILAGE TISSUE: REGULATION OF CHONDROCYTE PROLIFERATION, GEROPROTECTION AND PROSPECTS FOR USE IN OSTEOARTHROSIS

DOI: https://doi.org/10.29296/25877305-2023-10-08
Issue: 
10
Year: 
2023

S. Myakisheva(1); Professor N. Linkova(1); Biol.D; Professor V. Polyakova(1, 2), Professor of the Russian Academy of Sciences, Biol.D; Professor G. Ryzhak(1); MD, Honored Scientist of the Russian Federation
1-Saint Petersburg Institute of Bioregulation and Gerontology
2-Saint-Petersburg State Research Institute of Phthisiopulmonology, Ministry
of Health of Russia

Objective. To evaluate the effect of a polypeptide complex isolated from bovine cartilage tissue (PPCC) and its constituent AED peptide on the proliferation of chondrocytes, obtained from young and old rats. Materials and methods. Chondrocytes primary culture of young (3 months) and old (20 months) rats was used in the work. Cell proliferation on the 4th passage was evaluated by the cell growth curve for 5 days. PPCC and the AED peptide were studied at concentrations of 20; 200 and 2000 ng/ml. Results. The AED peptide at an effective concentration of 200 ng/ml increased the number of chondrocytes in cultures obtained from young and old animals by 1.4–1.8 and 1.6–2.1 times, respectively, compared with the control. PPCC at an effective concentration of 2000 ng/ml stimulated the proliferation of chondrocytes in cultures obtained from young and old rats, respectively, by 1.7–2.2 and 1.8–2.5 times compared with the control. Conclusion. PPCC and the AED peptide have gero- and chondroprotective properties. Both peptides are more effective when acting on chondrocytes obtained from old animals. The obtained data open up prospects for the study of PPCC and the AED peptide as stimulators of regeneration and proliferation of chondrocytes in steoarthrosis models.
Keywords: 
cartilage polypeptide complex
AED peptide
chondrocytes
proliferation
aging
osteoarthrosis.

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