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Age-related aspects of the sirtuin expression level in the cardiomyocytes of patients with dilated cardiomyopathy

DOI: https://doi.org/10.29296/25877305-2022-10-14

K. Kravchenko(1); Professor K. Kozlov(1, 2), MD; Professor V. Polyakova(3, 4), Biol.D; Professor D. Medvedev(1, 5), MD
1-Saint Petersburg Institute of Bioregulation and Gerontology
2-S.M. Kirov Military Medical Academy, Saint Petersburg
3-Saint Petersburg Research Institute of Phthisiopulmonology, Ministry of Health of Russia
4-Belgorod State National Research University,
5-Research Institute of Hygiene, Occupational Diseases, and Human Ecology, Federal Biomedical Agency of Russia, Saint Petersburg

Sirtuins are among the signaling molecules that may have important prognostic value in dilated cardiomyopathy (DCM). Objective. To study the expression level of sirtuins in the cardiomyocytes of patients with DCM in vitro. Subjects and methods. The study used cardiomyocyte cultures taken during heart biopsy from 3 middle-aged male patients (mean age 52.3±2.6 years) with DCM. A culture of normal human cardiomyocytes served as a control. The investigators applied a primary dissociated cell culturing method and immunofluorescence confocal laser scanning microscopy. To simulate cellular senescence, they employed Passages 3 and 10 cells that corresponded to young and old cultures. Results. At the molecular level, cardiomyocyte aging was accompanied by a decrease in the expression of sirtuins 1, 3, and 6; whereas the expression of sirtuin 2 increased significantly in the old cultures versus the young ones in both the control and DCM groups. The findings suggest may suggest that sirtuins 1, 2, 3, and 6 are involved not only in the pathogenesis of DCM, but also in the mechanisms of aging.

dilated cardiomyopathy
cardiac cell aging
cell culture
confocal microscopy

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