Organizational approaches to optimizing immunochemical methods for the diagnosis of age-associated and vascular diseases

DOI: https://doi.org/10.29296/25877305-2022-05-06
Issue: 
5
Year: 
2022

U. Saginbaev, Candidate of Biological Sciences;
S. Rukavishnikova, Biol.D.; T. Akhmedov, Candidate of Medical Sciences
Saint Petersburg Institute of Bioregulation and Gerontology

Immunochemical methods play an important role in clinical and research studies. When studying the pathogenesis of age-associated vascular diseases, it often becomes necessary to investigate several analytes. Objective: to elaborate an algorithm for optimizing enzyme-linked immunosorbent assay (ELISA) studies with a parallel comprehensive assessment of several indicators determined in the investigation of age-associated and vascular diseases, atherosclerosis in particular. The materials were test systems for ELISA of a number of indicators characterizing the state of immunity, vascular wall, and metabolism. The duration of the study is one of the problems of immunochemical analysis of analyte panels. To solve this problem, the authors propose a parallel approach to conducting ELISA, which makes it possible to save about 16 hours. With this approach, the target analytes are grouped by the duration and multiplicity of incubation, the number of calibrators and other criteria. Thus, the described logistic principle that is able to substantially optimize a immunochemical study can be successfully applied in the comprehensive assessment of the causal relationships between age-associated and vascular diseases.
Keywords: 
immunochemistry
analyte panels
age
vascular disease
logistics.

References: 
  1. Karpishchenko A.I. Medical laboratory technologies: a guide to clinical laboratory diagnostics: in 2 tons/M.: GEOTAR-Media, 2013; 792 p. (in Russ.).
  2. Kamyshnikov V.S. Clinical Laboratory Research Methods/M.: MEDPress-inform, 2020; 736 p. (in Russ.).
  3. Bureneva O.I., Muravnik L.M., Safyannikov I.N. Plate analyzer for Russian regions. Biotekhnosfera. 2015; 5 (41): 26–31 (in Russ.).
  4. Agarkov N.M., Markelov M.Yu., Markelova E.A. Laboratory diagnostics of ovarian cancer among the elderly based on the parameters of immunoglobulins and phagocytic activity of blood. Advances in gerontology. 2020; 1 (33): 82–6 (in Russ.). DOI: 10.34922/AE.2020.33.1.010
  5. Dedov D.V., Mazaev V.P., Ryazanova S.V. et al. The study of life quality in interventional and conservative treatments of the patients with stable angina at delayed observations. Cardiovascular Therapy and Prevention. 2014; 13 (5): 31–5 (in Russ.). DOI: 10.15829/1728-8800-2014-5-31-35
  6. Barbarash O.L., Kashtalap V.V., Zykov M.V. Association of osteopontin level with coronary atherosclerosis and osteoporosis in male patients with stable coronary artery disease. Atherosclerosis and dyslipidemia. 2016; 4 (25): 40–8 (in Russ.).
  7. Khabibulina M.M. Narusheniya ritma i provodimosti u zhenshchin s gipertonicheskoi bolezn’yu v period premenopauzy v zavisimosti ot funktsii endoteliya perifericheskikh arterii. Ural’skii meditsinskii zhurnal. 2008; 2 (42): 28–31 (in Russ.)
  8. Duda-Sobczak A., Falkowski B., Araszkiewicz A. et al. Accociation Between Self-Reported Physical Activity and Skin Autoflorescence, a Marker of Tissue Accumulation of Advanced Glycation End Products in Adults with Type 1 Diabetes: A cross-sectional Stady. Clin Ther. 2018; 40 (6): 872–80. DOI: 10.1016/j.clinthera.2018.02.016
  9. Kandola A., Ashdown-Franks G., Hendrikse J. et al. Physical activity and depression: Towards understanding the antidepressant mechanisms of physical activity. Neurosci Biobehav Rev. 2019; 107: 525–39. DOI: 10.1016/j.neubiorev.2019.09.040
  10. Bonavolonta V., Greco F., Sabatini U. et al. Effects of Ballroom Dance on Physical Fitness and Reaction Time in Experienced Middle-Aged Adults of Both Genders. Int J Environ Res Public Health. 2021; 18 (4): 2036. DOI: 10.3390/ijerph18042036
  11. Neufer P.D., Bamman M.M., Muoio D.M. et al. Understanding the Cellular and Molecular Mechanisms of Physical Activity – Induced Helth Benefits. Cell Metabolism. 2015; 22 (1): 4–11. DOI: 10.1016/j.cmet.2015.05.011
  12. Pilch W., Tota L., Piotrowska A. et al. Effect of Nordic Walking on Oxidant and Antioxidant Status: Levels of Calcidol and Proinflammatory Cytokines in Middle-Aged Women. Oxid Med Cell Longev. 2018: 6468234. DOI: 10.1155/2018/6468234
  13. Hackney M.E., Earhart G.M. Effects of dance on movement control in Parkinson's disease: a comparison of Argentine tango and American ballroom. J Rehabil Med. 2009; 41 (6): 475–81. DOI: 10.2340/16501977-0362
  14. Teixeira-Machado L., Arida R.M., de Jesus Mari J. Dance for neuroplasticity: A descriptive systematic review. Neurosci Biobehav Rev. 2019; 96: 232–40. DOI: 10.1016/j.neubiorev.2018.12.010
  15. Karpati F.J., Giacosa C., Foster N.E. et al. Dance and the brain: a review. Ann N Y Acad Sci. 2015; 1337: 140–6. DOI: 10.1111/nyas.12632
  16. Pereira A.P.S., Marinho V., Gupta D. et al. Music Therapy and Dance as Gait Rehabilitation in Patients With Parkinson Disease: A Review of Evidence. J Geriatr Psychiatry Neurol. 2019; 32 (1): 49–56. DOI: 10.1177/0891988718819858
  17. Murcia C.Q., Bongard S., Kreutz G. Emotional and Neurohumoral Responses to Dancing Tango Argentino: The Effects of Music and Partner. Music and Medicine. 2009; 1: 14–21.
  18. Ho R.T.H., Fong T.C.T., Yip P.S.F. Perceived stress moderates the effects of a randomized trial of dance movement therapy on diurnal cortisol slopes in breast cancer patients. Psychoneuroendocrinology. 2018; 87: 119–26. DOI: 10.1016/j.psyneuen.2017.10.012
  19. Rodrigues-Krause J., Krause M., Reischak-Oliveira A. Dancing for Healthy Aging: Functional and Metabolic Perspectives. Altern Ther Health Med. 2019; 25 (1): 44–63.
  20. Piazza J.R., Dmitrieva N.O., Charles S.T. et al. Diurnal cortisol profiles, inflammation, and functional limitations in aging: Findings from the MIDUS study. Health Psychol. 2018; 37 (9): 839–49. DOI: 10.1037/hea0000629
  21. Karkou V., Meekums B. Dance movement therapy for dementia. Cochrane Database Syst Rev. 2017; 2 (2): CD011022. DOI: 10.1002/14651858.CD011022.pub2
  22. Vrinceanu T., Esmail A., Berryman N. et al. Dance your stress away: comparing the effect of dance/movement training to aerobic exercise training on the cortisol awakening response in healthy older adults. Stress. 2019; 22 (6): 687–95. DOI: 10.1080/10253890.2019.1617690
  23. Fernandez-Arguelles E.L., Rodriguez-Mansilla J., Antunez L.E. et al. Effects of dancing on the risk of falling related factors of healthy older adults: a systematic review. Arch Gerontol Geriatr. 2015; 60 (1): 1–8. DOI: 10.1016/j.archger.2014.10.003
  24. Koehne S., Schmidt M.J., Dziobek I. The role of interpersonal movement synchronisation in empathic functions: Insights from Tango Argentino and Capoeira. Int J Psychol. 2016; 51 (4): 318–22. DOI: 10.1002/ijop.12213
  25. Lakes K.D., Marvin S., Rowley J. et al. Dancer perceptions of the cognitive, social, emotional, and physical benefits of modern styles of partnered dancing. Complement Ther Med. 2016; 26: 117–22. DOI: 10.1016/j.ctim.2016.03.007
  26. Roberge R.J., Kim J.H., Yorio P. et al. Umbilical temperature correlation with core and skin temperatures at rest, in the heat and during physical activity. J Int Hypertermia. 2017; 33 (7): 724–32. DOI: 10.1080/02656736.2017.1315180
  27. Jeong J.-Y., Hong S.-H., Lee M.S. et al. Dance movement therapy improves emotional responses and modulates neurohormones in adolescents with mild depression. Int J Neurosci. 2005; 115 (12): 1711–20. DOI: 10.1080/00207450590958574
  28. Karpati F.J., Giacosa C., Foster N.E.V. et al. Structural Covariance Analysis Reveals Differences Between Dancers and Untrained Controls. Front Hum Neurosci. 2018; 12: 373. DOI: 10.3389/fnhum.2018.00373
  29. Evtyukhin I.Y., Ryazanova S.V., Dedov D.V. et al. Comparison of quality-of-life parameters in the long-term period of interventional treatment in patients with coronary heart disease. Modern problems of science and education. 2015; 6: 145 (in Russ.). DOI: 10.17513/spno.23336