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Organizational approaches to optimizing immunochemical methods for the diagnosis of age-associated and vascular diseases

DOI: https://doi.org/10.29296/25877305-2022-05-06

U. Saginbaev, Candidate of Biological Sciences;
S. Rukavishnikova, Biol.D.; T. Akhmedov, Candidate of Medical Sciences
Saint Petersburg Institute of Bioregulation and Gerontology

Immunochemical methods play an important role in clinical and research studies. When studying the pathogenesis of age-associated vascular diseases, it often becomes necessary to investigate several analytes. Objective: to elaborate an algorithm for optimizing enzyme-linked immunosorbent assay (ELISA) studies with a parallel comprehensive assessment of several indicators determined in the investigation of age-associated and vascular diseases, atherosclerosis in particular. The materials were test systems for ELISA of a number of indicators characterizing the state of immunity, vascular wall, and metabolism. The duration of the study is one of the problems of immunochemical analysis of analyte panels. To solve this problem, the authors propose a parallel approach to conducting ELISA, which makes it possible to save about 16 hours. With this approach, the target analytes are grouped by the duration and multiplicity of incubation, the number of calibrators and other criteria. Thus, the described logistic principle that is able to substantially optimize a immunochemical study can be successfully applied in the comprehensive assessment of the causal relationships between age-associated and vascular diseases.

analyte panels
vascular disease

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