HAC (Russian)
RSCI (Russian)
Ulrichsweb (Ulrich’s Periodicals Directory)
Scientific Indexing Services

Efficiency of isolation of stromal vascular fraction cells from lipoaspirate without enzymatic treatment

DOI: https://doi.org/10.29296/25877305-2019-08-05

A. Mishra(1, 2); A. Semchenkova(2), V. Zhogov(2), A. Bader(3), A. Melerzanov(1), Candidate of Medical Sciences 1-Moscow Institute of Physics and Technology 2-Dmitry Rogachev National Medical Research Center of Pediatric Hematology, Oncology, and Immunology, Moscow 3-University of Leipzig, Germany

This study is a comparative analysis of methods for obtaining a stromal vascular fraction from lipoaspirate. The first method is the classical isolation of a cell population using enzymatic treatment of adipose tissue in a cell laboratory. The second method can be implemented in the operating room, without using enzymes, and within a short time.

adipose tissue-derived mesenchymal stem cells
stromal vascular fraction
skin care
regenerative cosmetology

  1. American Society of Plastic Surgeons. New Statistics Reveal the Shape of Plastic Surgery. GlobeNewswire News Room / GlobeNewswire, 1 Mar. 2018, www.globenewswire.com/news-release/2018/03/01/1402022/0/en/New-Statistics-Reveal-the-Shape-of-Plastic-Surgery.html/
  2. Anonymous. Regulations, Directives and Other Acts / European Union, 7 Mar. 2019, www.europa.eu/european-union/eu-law/legal-acts_en
  3. Anonymous. Advanced Therapy Medicinal Products: Overview / European Medicines Agency, 16 Mar. 2019, www.ema.europa.eu/en/human-regulatory/overview/advanced-therapy-medicinal-products-overview
  4. Peng G. Platelet-Rich Plasma for Skin Rejuvenation: Facts, Fiction, and Pearls for Practice // Facial Plastic Surgery Clinics. – 2019; 27 (3): 405–11. DOI: 10.1016/j.fsc.2019.04.006.
  5. Gir Ph. et al. Human adipose stem cells: current clinical applications // Plastic and reconstructive surgery. – 2012; 129 (6): 1277–90. DOI: 10.1097/PRS.0b013e31824ecae6
  6. Charles-de-Sá L. et al. Antiaging treatment of the facial skin by fat graft and adipose-derived stem cells // Plastic and reconstructive surgery. – 2015; 135 (4): 999–1009. DOI: 10.1097/PRS.0000000000001123.
  7. Doi K. et al. Stromal vascular fraction isolated from lipo-aspirates using an automated processing system: bench and bed analysis // J. Tissue Eng. Regen. Med. – 2013; 7 (11): 864–70. DOI: 10.1002/term.1478