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THE QUESTION OF PATHOGENESIS SEPTIC COMPLICATIONS IN CALCULOUS CHOLECYSTITISAND DEVELOPMENT OF METHODS FOR THEIR PREVENTION

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Issue: 
7
Year: 
2015

R. Andreev (1,4); Professor Yu. Vinnik (1), MD; E. Serova (1,3), Candidate of Medical Sciences; L. Didenko (2), MD; N. Shevlyagina (2), Candidate of Medical Sciences; I. Prusov (3); A. Shirobokov (3); D. Zdzitovetsky (1,4), MD; S. Maslov (4); P. Bazarin (4); A. Kovalev (4) 1 -Krasnoyarsk State Medical University named after Prof. V.F. Voyno-Yasenetsky 2 -Scientific Research Institute of Epidemiology and Microbiology named after N.F. Gamaleya 3 -Krasnoyarsk Interdistrict Hospital №4 4 -Krasnoyarsk Interdistrict Hospital ambulance named after N.S. Karpovich

In the present study performed electron microscopic examination of gallstones and the surface of the epithelial lining of the gallbladder in destructive forms of calculouscholecystitis and found biofilm microorganisms. Significantly more bacteria were detected inside the stone, the vast majority of whom were vegetative forms. Biofilms mainly localized on the surface of the stone, which is obviously due to the adverse effects of bile bacteria and outer cell matrixof the biofilmis a protective barrier against external aggressive environment for bacteria. These provisions may be used in the correction of preoperative and postoperative therapy in patients with cholelithiasis, acute calculouscholecystitis with pathogenetic point of view. One of the cardinal directions of the future in the prevention of complications of gallstone disease should be to develop techniques capable of destroying outer cell matrix of the biofilms.

Keywords: 
cholelithiasis
acute calculouscholecystitis
biofilm microorganisms
electron microscopic examination



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