Investigation of β-defensin-2 in breast milk in infant atopic dermatitis

DOI: https://doi.org/10.29296/25877305-2020-05-20
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Issue: 
5
Year: 
2020

Yu. Bunina (Boguslavskaya)1; Professor A. Kudryavtseva(1, 2), MD; E. Bystritskaya(2); G. Usatova(2); K. Levina(1); O. Svitich(1, 2) (1)I.M. Sechenov First Moscow State Medical University (Sechenov University) (2)I.I. Mechnikov Research Institute for Vaccines and Sera, Moscow

Purpose. To assess the link between β-defensin-2concentration in breastmilk with the severity of AD in its debut in infants and young children. Materials and methods. 25 samples of breastmilk were analyzed. 2 groups were selected. The first group included 14 samples from mothers whose children got AD in the last 6 months: mild AD and moderate AD. Analysis of breastmilk samples by the enzyme-linked immunosorbent assay was conducted to find the β-defensin-2 concentration. ELISA Kit for β-Defensin-2 (DEFb2) was used. Results and discussion. We have showed that in the breastmilk of healthy children mothers a background level of the β-defensin 2 is detected (209,6 pg/ml). In the case of mild AD the concentration of β-defensin 2 was lower (HBD-2 – 166,3 pg/ml. In breastmilk of mothers whose children had moderate AD the level of β-defensin-2 concentration was significantly lower than in the first group (HBD-2 – 71,2 pg/ml). Thus we can make a conclusion that the decrease of the β-defensin-2 concentration in the breastmilk can be one of prognostic criteria of more severe AD in infants and young children.
Keywords: 
defensin
breastmilk
atopic dermatitis
innate immunity

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References: 
  1. Wollenberg A., Barbarot S., Bieber T. et al. Consensus based European guidelines for treatment of atopic eczema (atopic dermatitis) in adults and children: part I. J Eur Acad Dermatol Venereol. 2018; 32: 657–82. DOI: 10.1111/jdv.14891
  2. Niyonsaba F., Nagaoka I., Ogawa H. et al. Multifunctional antimicrobial proteins and peptides: natural activators of immune systems. Curr Pharm Des. 2009; 15 (21): 2393–413. DOI: 10.2174/138161209788682271
  3. Lee S.H., Jeong S.K., Ahn S.K. An update of the defensive barrier function of skin. Yonsei Med J. 2006; 47: 293–306. DOI: 10.3349/ymj.2006.47.3.293
  4. Kudrjavtseva A.V., Katosova L.K., Balabolkin I.P. i dr. Rol' zolotistogo stafilokokka pri atopicheskom dermatite u detej. Pediatrija. Zhurnal im. G.N. Speranskogo. 2003; 6 (82): 32–6 [Kudryavtseva A.V., Katosova L.K., Balabolkin I.I. et al. Role of Staphylococcus aureus in pediatric atopic dermatitis. Pediatria. Journal named after G.N.Speransky. 2003; 82 (6): 32–6 (in Russ.)].
  5. Fleischer D.M., Spergel J.M., Assa A.H. et al. Primary prevention of allergic disease through nutritional interventions. J Allergy Clin Immunol Pract. 2008; 1: 29–36. DOI: 10.1016/j.jaip.2012.09.003
  6. Gdalevich M., Mimouni D., David M. et al. Breast-feeding and the onset of atopic dermatitis in childhood: A systematic review and meta-analysis of prospective studies. J Am Acad Dermatol. 2001; 45: 520–7. DOI: 10.1067/mjd.2001.114741
  7. Ito J., Fujiwara T. Breastfeeding and risk of atopic dermatitis up to the age 42 months: A birth cohort study in Japan. Ann Epidemiol. 2014; 24: 267–72. DOI: 10.1016/j.annepidem.2013.11.007
  8. Munblit D., Treneva M., Peroni D.G. et al. Immune Components in Human Milk Are Associated with Early Infant Immunological Health Outcomes: A Prospective Three-Country Analysis. Nutrients. 2017; 9 (6): 532. DOI: 10.3390/nu9060532
  9. Jakaitis B.M., Denning P.W. Human breast milk and the gastrointestinal innate immune system. Clin Perinatol. 2014; 41 (2): 423–35. DOI: 10.1016/j.clp.2014.02.011
  10. Hakansson A.P. Protective effects of human milk antimicrobial peptides against bacterial infection. J Pediatr (Rio J.). 2015; 91 (1): 4–5. https://doi.org/10.1016/j.jped.2014.10.001
  11. Metz-Boutigue M.-H., Shooshtarizadeh P., Prevost G. et al. Antimicrobial Peptides Present in Mammalian Skin and Gut are Multifunctional Defence Molecules. Curr Pharm Des. 2010; 16: 1024. https://doi.org/10.2174/138161210790963823,
  12. Tunzi C.R., Harper P.A., Bar-Oz B. et al. Beta-defensin expression in human mammary gland epithelia. Pediatr Res. 2000; 48: 30–5. DOI: 10.1203/00006450-200007000-00008
  13. Baricelli J., Rocafull M.A., Vázquez D. et al. β-Defensin-2 in breast milk displays a broad antimicrobial activity against pathogenic bacteria. J Pediatr (Rio J.). 2015; 91 (1): 36–43. https://doi.org/10.1016/j.jped.2014.05.006
  14. Jing Wu, Ruo-Lan Gong, Qing-Feng Hu et al. Immunoregulatory effect of human β-defensin 1 on neonatal cord blood monocyte-derived dendritic cells and T cells. Mol Immunol. 2019; 109: 99–107. https://doi.org/10.1016/j.molimm.2019.03.007.
  15. Savilahti E.M., Kukkonen A.K., Kuitunen M., et al. Soluble CD14, α- and β-defensins in breast milk: Association with the emergence of allergy in a high-risk population. Innate Immunity. 2015; 21 (3): 332–7. https://doi.org/10.1177/1753425914541560
  16. Rachid R., Chatila T.A. The role of the gut microbiota in food allergy. Curr Opin Pediatr. 2016; 6 (28): 748–75. DOI: 10.1097/MOP.0000000000000427